Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells

Gene. 1990 Feb 14;86(2):263-8. doi: 10.1016/0378-1119(90)90288-3.

Abstract

Gene expression in heterologous species is a powerful tool for the cloning and characterization of genes. Here, we present a family of expression shuttle vectors which work in the budding yeast, Saccharomyces cerevisiae, and also in mammalian cells. The quantity of product expressed by the gene under study can be modulated in yeast by virtue of the control over plasmid copy number and culture conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Codon / genetics
  • Gene Expression*
  • Genetic Techniques
  • Genetic Vectors*
  • L Cells / enzymology
  • Mice
  • Molecular Sequence Data
  • Plasmids*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Codon
  • beta-Galactosidase