Characteristics of high-molecular-weight hyaluronic acid as a brain-derived neurotrophic factor scaffold in periodontal tissue regeneration

Tissue Eng Part A. 2011 Apr;17(7-8):955-67. doi: 10.1089/ten.TEA.2010.0070. Epub 2011 Jan 16.

Abstract

Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor / chemistry*
  • Brain-Derived Neurotrophic Factor / pharmacology
  • Cattle
  • Cell Adhesion / genetics
  • Cell Adhesion / physiology
  • Cells, Cultured
  • Humans
  • Hyaluronic Acid / chemistry*
  • Hyaluronic Acid / pharmacology
  • Immunohistochemistry
  • Microscopy, Fluorescence
  • Molecular Weight
  • Periodontium / cytology*
  • Regeneration / drug effects
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Scaffolds / chemistry*

Substances

  • Brain-Derived Neurotrophic Factor
  • Hyaluronic Acid