Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals

Mol Cell Biol. 1990 May;10(5):1931-9. doi: 10.1128/mcb.10.5.1931-1939.1990.


Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Division
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • Epidermal Growth Factor / pharmacology
  • Gene Expression Regulation / drug effects
  • Immunologic Techniques
  • Metalloproteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Nerve Growth Factors / pharmacology
  • Nuclear Proteins / physiology*
  • Phosphoproteins / genetics
  • Protein Processing, Post-Translational
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transcription Factors / genetics*
  • Zinc / physiology


  • DNA-Binding Proteins
  • Metalloproteins
  • Nerve Growth Factors
  • Nuclear Proteins
  • Phosphoproteins
  • Transcription Factors
  • Epidermal Growth Factor
  • Zinc
  • Tetradecanoylphorbol Acetate