Hamster embryo cells transformed by herpes simplex virus: reactions with adeno-associated satellite virus (AAV) and its adenovirus helpers

Cancer Biochem Biophys. 1977;2(1):25-31.


We have studied the reactions of hamster embryo cells transformed by ultraviolet-inactivated herpes simplex type 2 (333-8-9 T cells) to infections with adeno-associated satellite virus (AAV) and its adenovirus helpers. Resident HSV structural antigens were not detectable in early or late passage of 333-8-9 T cells. AAV structural antigens were not detected in these cells unless the cells were coinfected with a helper adenovirus. In early passage 333-8-9 T cells were permissive to infections with simian adenovirus SV15 whereas normal hamster cell line LSH was nonpermissive. In some late passages of 333-8-9 T cells infections with SV15 adenovirus led to the production of viruslike particles whose morphology was identical with reoviruses.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae*
  • Adenoviruses, Human
  • Adenoviruses, Simian
  • Animals
  • Antigens, Viral
  • Cell Transformation, Neoplastic*
  • Cricetinae
  • Dependovirus* / immunology
  • Genes, Viral
  • Helper Viruses
  • Inclusion Bodies, Viral
  • Microscopy, Electron
  • Simplexvirus* / genetics
  • Simplexvirus* / immunology


  • Antigens, Viral