NG2+ CNS Glial Progenitors Remain Committed to the Oligodendrocyte Lineage in Postnatal Life and Following Neurodegeneration

Neuron. 2010 Nov 18;68(4):668-81. doi: 10.1016/j.neuron.2010.09.009.

Abstract

The mammalian CNS contains a ubiquitous population of glial progenitors known as NG2+ cells that have the ability to develop into oligodendrocytes and undergo dramatic changes in response to injury and demyelination. Although it has been reported that NG2+ cells are multipotent, their fate in health and disease remains controversial. Here, we generated PDGFαR-CreER transgenic mice and followed their fate in vivo in the developing and adult CNS. These studies revealed that NG2+ cells in the postnatal CNS generate myelinating oligodendrocytes, but not astrocytes or neurons. In regions of neurodegeneration in the spinal cord of ALS mice, NG2+ cells exhibited enhanced proliferation and accelerated differentiation into oligodendrocytes but remained committed to the oligodendrocyte lineage. These results indicate that NG2+ cells in the normal CNS are oligodendrocyte precursors with restricted lineage potential and that cell loss and gliosis are not sufficient to alter the lineage potential of these progenitors.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Differentiation / genetics
  • Cell Lineage* / genetics
  • Cell Proliferation
  • Disease Models, Animal
  • Mice
  • Mice, Transgenic
  • Nerve Degeneration / genetics
  • Nerve Degeneration / metabolism
  • Nerve Degeneration / pathology*
  • Neurogenesis / genetics
  • Neuroglia / cytology*
  • Neuroglia / metabolism
  • Neuroglia / ultrastructure
  • Oligodendroglia / cytology*
  • Oligodendroglia / pathology
  • Oligodendroglia / ultrastructure
  • Random Allocation
  • Receptor, Platelet-Derived Growth Factor alpha / genetics
  • Receptor, Platelet-Derived Growth Factor alpha / physiology
  • Spinal Cord / cytology
  • Spinal Cord / metabolism
  • Spinal Cord / ultrastructure
  • Stem Cells / cytology*
  • Stem Cells / pathology
  • Stem Cells / ultrastructure

Substances

  • Receptor, Platelet-Derived Growth Factor alpha