Placental microRNA Expression in Pregnancies Complicated by Preeclampsia

Am J Obstet Gynecol. 2011 Feb;204(2):178.e12-21. doi: 10.1016/j.ajog.2010.09.004. Epub 2010 Nov 20.

Abstract

Objective: The role of posttranscription regulation in preeclampsia is largely unknown. We investigated preeclampsia-related placental microRNA (miRNA) expression using microarray and confirmatory quantitative real-time polymerase chain reaction experiments.

Study design: Placental expressions of characterized and novel miRNAs (1295 probes) were measured in samples collected from 20 preeclampsia cases and 20 controls. Differential expression was evaluated using Student t test and fold change analyses. In pathway analysis, we examined functions/functional relationships of targets of differentially expressed miRNAs.

Results: Eight miRNAs were differentially expressed (1 up-regulated and 7 down-regulated) among preeclampsia cases compared with controls. These included previously identified candidates (miR-210, miR-1, and a miRNA in the 14q32.31 cluster region) and others that are novel (miR-584 and miR-34c-5p). These miRNAs target genes that participate in organ/system development (cardiovascular and reproductive system), immunologic dysfunction, cell adhesion, cell cycle, and signaling.

Conclusion: Expression of miRNAs that target genes in diverse pathophysiological processes is altered in the setting of preeclampsia.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Female
  • Gene Expression
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Placenta / metabolism*
  • Pre-Eclampsia / genetics
  • Pre-Eclampsia / metabolism*
  • Pregnancy
  • Principal Component Analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • MicroRNAs