A C/T mutation in microRNA target sites in BMP5 gene is potentially associated with fatness in pigs

Meat Sci. 2011 Mar;87(3):299-303. doi: 10.1016/j.meatsci.2010.09.013. Epub 2010 Oct 30.

Abstract

Bone morphogenetic protein 5 (BMP5) gene was suggested to be a potential positional candidate gene for fatness trait QTLs on SSC7. Here by comparative sequencing of BMP5 gene in Meishan (MS) and Large White (LW) pigs, one SNP *131C>T in 3' un-translated region was detected. Association analysis results in 322 LW×MS F(2) pigs showed that CC pigs had thinner back fatness and heavier ham than TC (P<0.05 or P<0.1), and had a higher fat percentage and a lower lean meat percentage (P<0.1) than TT. Moreover, this C/T transition was predicted to alter BMP5 interaction with let-7c and miR-184 by using RNA22 and RNAhybrid. The negative expression of BMP5 gene with let-7c and miR-184 detected from miRNAs overexpression in swine fibroblast, indicating these 2 miRNAs might participate in the translational inhibition of BMP5 gene. Overall, SNP *131C>T might be a good marker for fatness traits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adiposity / genetics
  • Algorithms
  • Animals
  • Binding Sites
  • Body Composition / genetics*
  • Bone Morphogenetic Protein 5 / genetics*
  • Bone Morphogenetic Protein 5 / metabolism
  • Cells, Cultured
  • Female
  • Fibroblasts
  • Gene Frequency
  • Genetic Association Studies / veterinary
  • Genetic Markers
  • Male
  • MicroRNAs / metabolism*
  • Models, Chemical
  • Mutation
  • Polymorphism, Single Nucleotide*
  • Quantitative Trait Loci / genetics
  • Sus scrofa / genetics*
  • Sus scrofa / growth & development*

Substances

  • 3' Untranslated Regions
  • Bone Morphogenetic Protein 5
  • Genetic Markers
  • MicroRNAs