Cytotoxicity of δ-tocotrienols from Kielmeyera coriacea against cancer cell lines

Bioorg Med Chem. 2011 Jan 1;19(1):623-30. doi: 10.1016/j.bmc.2010.10.044. Epub 2010 Oct 25.


In the search for new anti-cancer compounds, Brazilian Cerrado plant species have been investigated. The hexane root bark extract of Kielmeyera coriacea lead to a mixture of δ-tocotrienol (1) and its dimer (2). The structures of both compounds 1 and 2 were established based on detailed 1D and 2D NMR and EI-MS analyses. The cytotoxicity of the mixture was tested against four human tumor cell lines in the following cultures: MDA-MB-435 (melanoma), HCT-8 (colon), HL-60 (leukemia), and SF-295 (glioblastoma), and displayed IC(50) values ranging from 8.08 to 23.58μg/mL. Additional assays were performed in order to investigate the mechanism of action of the mixture (1+2) against the human leukemia cell line HL-60. The results suggested that the mixture suppressed leukemia growth and reduced cell survival, triggering both apoptosis and necrosis, depending on the concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Cell Cycle / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • DNA Replication / drug effects
  • Drug Screening Assays, Antitumor
  • Humans
  • Magnetic Resonance Spectroscopy
  • Magnoliopsida / chemistry*
  • Microscopy, Fluorescence
  • Spectrometry, Mass, Electrospray Ionization
  • Tocotrienols / pharmacology*


  • Antineoplastic Agents, Phytogenic
  • Tocotrienols