The same synaptic vesicles drive active and spontaneous release

Nat Neurosci. 2010 Dec;13(12):1454-6. doi: 10.1038/nn.2690.


Synaptic vesicles release neurotransmitter both actively (on stimulation) and spontaneously (at rest). It has been assumed that identical vesicles use both modes of release; however, recent evidence has challenged this view. Using several assays (FM dye imaging, pHluorin imaging and antibody-labeling of synaptotagmin) in neuromuscular preparations from Drosophila, frog and mouse, as well as rat cultured neurons, we found that the same vesicles participate in active and spontaneous release.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anura
  • Cells, Cultured
  • Drosophila melanogaster
  • Endocytosis / physiology*
  • Mice
  • Neuromuscular Junction / cytology
  • Neuromuscular Junction / metabolism
  • Neurons / cytology
  • Neurons / metabolism
  • Rats
  • Rats, Wistar
  • Species Specificity
  • Synaptic Transmission / physiology*
  • Synaptic Vesicles / metabolism*