A Robust System for Production of Minicircle DNA Vectors

Nat Biotechnol. 2010 Dec;28(12):1287-9. doi: 10.1038/nbt.1708. Epub 2010 Nov 21.

Abstract

Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Biotechnology / methods*
  • Cloning, Molecular / methods*
  • DNA, Circular*
  • Escherichia coli / genetics
  • Genetic Vectors*
  • Plasmids*

Substances

  • DNA, Circular