HPLC analysis of polyphenols in the fruits of Rubus idaeus L. (Rosaceae)

Nat Prod Res. 2010 Nov;24(19):1811-22. doi: 10.1080/14786411003754231.


The separation of anthocyanins present in the fruits of 11 varieties of red raspberries (Rubus idaeus L.) was performed by high performance liquid chromatography (HPLC) with a diode-array detector and evaporative light scattering detection (ELSD). The ELSD parameters--drift tube temperature, nebulising gas flow rate and gain value--were optimised to get the best detection and identification of the anthocyanins. The varieties Heritage and Willamette had the simplest anthocyanin sets consisting of only two predominant anthocyanins--cyanidin-3-O-sophoroside (1) and cyanidin-3-O-glucoside (3), while in the other varieties two other predominant compounds were also present, cyanidin-3-O-rutinoside (4) and cyanidin-3-O-(2(G)-O-glucosylrutinoside) (2). Moreover, using ELSD, simultaneous analysis of anthocyanins and sanguiin H-6 (5), an ellagitannin, was performed. The contents of anthocyanins and sanguiin H-6 (5) were estimated by HPLC with ultraviolet-visible (UV-vis) light detection. The determined concentrations of anthocyanins varied from 76.22 to 277.06 mg per 100 g of dry weight (d.w.). The content of sanguiin H-6 (5) was in the range from 135.04 to 547.48 mg per 100 g of d.w.

MeSH terms

  • Anthocyanins / chemistry
  • Chromatography, High Pressure Liquid*
  • Flavonoids / chemistry*
  • Fruit / chemistry*
  • Glucosides / chemistry
  • Molecular Structure
  • Phenols / chemistry*
  • Polyphenols
  • Rosaceae / chemistry*


  • Anthocyanins
  • Flavonoids
  • Glucosides
  • Phenols
  • Polyphenols
  • cyanidin-3-O-beta-glucopyranoside
  • cyanidin 3-rutinoside