Synthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity

Colloids Surf B Biointerfaces. 2011 Mar;83(1):16-22. doi: 10.1016/j.colsurfb.2010.10.033. Epub 2010 Oct 30.

Abstract

A solution-based approach to the synthesis of silver (Ag) nanoparticles by chemical reduction of AgNO(3) in a graphene oxide (GrO) suspension is demonstrated. X-ray diffraction and transmission electron microscopy indicate that the Ag nanoparticles, of size range 5-25nm, were decorated on the GrO sheets. The size and shape of the Ag nanoparticles are dependent on the concentration of the AgNO(3) solution. Antimicrobial activity of such hybrids materials is investigated against the Gram negative bacteria Escherichia coli and Pseudomonous aeruginosa. The bacterial growth kinetics was monitored in nutrient broth supplemented with the Ag nanoparticle-GrO suspension at different conditions. It was observed that P. aeruginosa is comparatively more sensitive to the Ag nanoparticle-GrO suspension.

MeSH terms

  • Anti-Infective Agents / chemical synthesis*
  • Anti-Infective Agents / pharmacology*
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Graphite / chemistry*
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Microbial Sensitivity Tests
  • Oxides / chemistry*
  • Particle Size
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / growth & development
  • Silver / chemistry*
  • Silver / pharmacology*
  • Spectrophotometry, Ultraviolet
  • Suspensions
  • X-Ray Diffraction

Substances

  • Anti-Infective Agents
  • Oxides
  • Suspensions
  • Silver
  • Graphite