Augmentation of regulatory B cell activity in experimental allergic encephalomyelitis by glatiramer acetate

J Neuroimmunol. 2011 Mar;232(1-2):136-44. doi: 10.1016/j.jneuroim.2010.10.031. Epub 2010 Nov 26.


We recently showed that B cells reduce CNS inflammation in mice with experimental allergic encephalomyelitis (EAE). Here, we demonstrate that adoptively transferred CD5/CD19+ B cells protect against EAE severity. Furthermore, we show that glatiramer acetate (GA), a therapeutic for relapsing multiple sclerosis treatment, amplifies this effect. Transfer of GA-conditioned B cells leads to increased production of immunoregulatory cytokines and reduced CNS inflammation, as well as decreased expression of the chemokine receptor, CXCR5, and elevated BDNF expression in the CNS. Thus B cells can protect against EAE, and GA augments this effect in maintaining immune homeostasis and controlling EAE disease progression.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigens, CD19 / immunology
  • Antigens, CD19 / metabolism
  • B-Lymphocyte Subsets / drug effects*
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocyte Subsets / metabolism
  • Brain-Derived Neurotrophic Factor / biosynthesis
  • Brain-Derived Neurotrophic Factor / drug effects
  • CD5 Antigens / immunology
  • CD5 Antigens / metabolism
  • Cell Separation
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Encephalomyelitis, Autoimmune, Experimental / metabolism
  • Encephalomyelitis, Autoimmune, Experimental / pathology
  • Female
  • Flow Cytometry
  • Glatiramer Acetate
  • Immunosuppressive Agents / pharmacology*
  • Mice
  • Mice, Inbred C57BL
  • Peptides / pharmacology*
  • Receptors, CXCR5 / biosynthesis
  • Receptors, CXCR5 / drug effects
  • Reverse Transcriptase Polymerase Chain Reaction


  • Antigens, CD19
  • Brain-Derived Neurotrophic Factor
  • CD5 Antigens
  • CXCR5 protein, mouse
  • Immunosuppressive Agents
  • Peptides
  • Receptors, CXCR5
  • Glatiramer Acetate