A novel real-time PCR method based on signaling-by-incorporation

Da-Rae Kim; Hee-Chul Ahn; Won-Ja Lee; Dae-Ro Ahn

doi:10.1039/c0cc04516k

A novel real-time PCR method based on signaling-by-incorporation

Chem Commun (Camb). 2011 Jan 14;47(2):791-3. doi: 10.1039/c0cc04516k. Epub 2010 Nov 26.

Authors

Da-Rae Kim¹, Hee-Chul Ahn, Won-Ja Lee, Dae-Ro Ahn

Affiliation

¹ Biomedical Research Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130-650, South Korea.

PMID: 21113520
DOI: 10.1039/c0cc04516k

Abstract

We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Deoxyguanine Nucleotides / chemistry*
Fluorine / chemistry*
Humans
Malaria / diagnosis
Plasmodium vivax / genetics
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 18S / metabolism

Substances

Deoxyguanine Nucleotides
RNA, Ribosomal, 18S
Fluorine
deoxyguanosine triphosphate