Jumonji domain protein JMJD5 functions in both the plant and human circadian systems

Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21623-8. doi: 10.1073/pnas.1014204108. Epub 2010 Nov 29.


Circadian clocks are near-ubiquitous molecular oscillators that coordinate biochemical, physiological, and behavioral processes with environmental cues, such as dawn and dusk. Circadian timing mechanisms are thought to have arisen multiple times throughout the evolution of eukaryotes but share a similar overall structure consisting of interlocking transcriptional and posttranslational feedback loops. Recent work in both plants and animals has also linked modification of histones to circadian clock function. Now, using data from published microarray experiments, we have identified a histone demethylase, jumonji domain containing 5 (JMJD5), as a previously undescribed participant in both the human and Arabidopsis circadian systems. Arabidopsis JMJD5 is coregulated with evening-phased clock components and positively affects expression of clock genes expressed at dawn. We found that both Arabidopsis jmjd5 mutant seedlings and mammalian cell cultures deficient for the human ortholog of this gene have similar fast-running circadian oscillations compared with WT. Remarkably, both the Arabidopsis and human JMJD5 orthologs retain sufficient commonality to rescue the circadian phenotype of the reciprocal system. Thus, JMJD5 plays an interchangeable role in the timing mechanisms of plants and animals despite their highly divergent evolutionary paths.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / physiology*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Biological Clocks / physiology*
  • Cell Line
  • Circadian Rhythm / physiology*
  • Gene Expression Regulation, Plant
  • Humans
  • Jumonji Domain-Containing Histone Demethylases / genetics
  • Jumonji Domain-Containing Histone Demethylases / metabolism*
  • Phenotype
  • Photoperiod
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism*
  • Seedlings / genetics
  • Seedlings / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism


  • Arabidopsis Proteins
  • Protein Isoforms
  • TOC1 protein, Arabidopsis
  • Transcription Factors
  • Jumonji Domain-Containing Histone Demethylases