Mutation of the Alzheimer's disease amyloid gene in hereditary cerebral hemorrhage, Dutch type

Science. 1990 Jun 1;248(4959):1124-6. doi: 10.1126/science.2111584.


An amyloid protein that precipitates in the cerebral vessel walls of Dutch patients with hereditary cerebral hemorrhage with amyloidosis is similar to the amyloid protein in vessel walls and senile plaques in brains of patients with Alzheimer's disease, Down syndrome, and sporadic cerebral amyloid angiopathy. Cloning and sequencing of the two exons that encode the amyloid protein from two patients with this amyloidosis revealed a cytosine-to-guanine transversion, a mutation that caused a single amino acid substitution (glutamine instead of glutamic acid) at position 22 of the amyloid protein. The mutation may account for the deposition of this amyloid protein in the cerebral vessel walls of these patients, leading to cerebral hemorrhages and premature death.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alleles
  • Alzheimer Disease / genetics*
  • Amino Acid Sequence
  • Amyloid / genetics*
  • Amyloid beta-Protein Precursor
  • Amyloidosis / complications
  • Amyloidosis / genetics*
  • Base Sequence
  • Brain Chemistry
  • Cerebral Hemorrhage / etiology
  • Cerebral Hemorrhage / genetics*
  • Cerebrovascular Disorders / complications
  • Cerebrovascular Disorders / genetics*
  • DNA
  • Deoxyribonucleases, Type II Site-Specific
  • Exons
  • Female
  • Genes, Dominant
  • Humans
  • Middle Aged
  • Molecular Sequence Data
  • Mutation*
  • Netherlands
  • Polymerase Chain Reaction
  • Protein Precursors / genetics*


  • Amyloid
  • Amyloid beta-Protein Precursor
  • Protein Precursors
  • DNA
  • endodeoxyribonuclease MboII
  • Deoxyribonucleases, Type II Site-Specific

Associated data

  • GENBANK/M37895
  • GENBANK/M37896