HIV drug resistance testing

Methods Mol Biol. 2011;665:123-32. doi: 10.1007/978-1-60761-817-1_8.

Abstract

HIV genotypic resistance testing has become a routine test informing the management of HIV-positive patients. This chapter describes the background of this testing and provides a protocol for undertaking the assay by a home-brew method. Methods for interpretation of the data are also outlined.

MeSH terms

  • Anti-Retroviral Agents / pharmacology
  • DNA, Viral / isolation & purification
  • Drug Resistance, Viral / genetics*
  • Genotype
  • HIV Infections / drug therapy*
  • HIV Infections / genetics
  • HIV Infections / virology*
  • HIV* / drug effects
  • HIV* / genetics
  • Humans
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA

Substances

  • Anti-Retroviral Agents
  • DNA, Viral