Exogenous coenzyme Q10 modulates MMP-2 activity in MCF-7 cell line as a breast cancer cellular model

Nutr J. 2010 Nov 30;9:62. doi: 10.1186/1475-2891-9-62.

Abstract

Background/aims: Matrix Metalloproteinases 2 is a key molecule in cellular invasion and metastasis. Mitochondrial ROS has been established as a mediator of MMP activity. Coenzyme Q(10) contributes to intracellular ROS regulation. Coenzyme Q(10) beneficial effects on cancer are still in controversy but there are indications of Coenzyme Q(10) complementing effect on tamoxifen receiving breast cancer patients.

Methods: In this study we aimed to investigate the correlation of the effects of co-incubation of coenzyme Q10 and N-acetyl-L-cysteine (NAC) on intracellular H2O2 content and Matrix Metalloproteinase 2 (MMP-2) activity in MCF-7 cell line.

Results and discussion: Our experiment was designed to assess the effect in a time and dose related manner. Gelatin zymography and Flowcytometric measurement of H2O2 by 2'7',-dichlorofluorescin-diacetate probe were employed. The results showed that both coenzyme Q10 and N-acetyl-L-cysteine reduce MMP-2 activity along with the pro-oxidant capacity of the MCF-7 cell in a dose proportionate manner.

Conclusions: Collectively, the present study highlights the significance of Coenzyme Q(10) effect on the cell invasion/metastasis effecter molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcysteine / pharmacology
  • Biomarkers / metabolism
  • Breast Neoplasms / metabolism*
  • Buthionine Sulfoximine / pharmacology
  • Carcinoma / metabolism*
  • Cell Line, Tumor
  • Down-Regulation / drug effects
  • Enzyme Inhibitors / pharmacology
  • Female
  • Free Radical Scavengers / pharmacology
  • Glutamate-Cysteine Ligase / antagonists & inhibitors
  • Humans
  • Hydrogen Peroxide / metabolism
  • Matrix Metalloproteinase 2 / metabolism*
  • Osmolar Concentration
  • Oxidation-Reduction
  • Oxidative Stress* / drug effects
  • Time Factors
  • Ubiquinone / analogs & derivatives*
  • Ubiquinone / metabolism

Substances

  • Biomarkers
  • Enzyme Inhibitors
  • Free Radical Scavengers
  • Ubiquinone
  • Buthionine Sulfoximine
  • Hydrogen Peroxide
  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • Glutamate-Cysteine Ligase
  • coenzyme Q10
  • Acetylcysteine