Lactobacillus acidophilus and Bifidobacterium bifidum stored at ambient temperature are effective in the treatment of acute diarrhoea

Ann Trop Paediatr. 2010;30(4):299-304. doi: 10.1179/146532810X12858955921159.


Introduction: Probiotics have demonstrated potential to reduce duration of diarrhoea and frequency of watery stools. Probiotics such as Lactobacillus acidophilus and Bifidobacterium bifidum (Infloran(®)) are usually maintained at a storage temperature of 4°C which is generally not feasible in tropical or sub-tropical countries.

Aim: The efficacy of Infloran(®) for treatment of acute diarrhoea when stored at 28-32°C (room temperature) was evaluated.

Methods: This was a double-blind, randomised study of infants and children aged 2 months to 7 years with acute diarrhoea. Patients were randomly assigned to receive Infloran(®) stored at 4°C, at room temperature, or to a placebo group. Duration of diarrhoea was a primary outcome, while the number of stools, hospital stay and requirement for rehydration fluid were secondary outcomes.

Results: Probiotics shortened duration of diarrhoea (34.1 and 34.8 hrs when stored either at 4°C or at room temperature, respectively, and 58 hrs with placebo, p<0.01) and reduced the number of stools (7.3 and 8 vs 15.9 with placebo, p<0.01).

Conclusion: Administration of probiotics is beneficial as additional treatment of acute diarrhoea and efficacy is not affected by storage temperature.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Bifidobacterium*
  • Child
  • Child, Preschool
  • Diarrhea / therapy*
  • Diarrhea, Infantile / therapy*
  • Double-Blind Method
  • Feces / microbiology
  • Female
  • Humans
  • Infant
  • Lactobacillus acidophilus*
  • Length of Stay
  • Male
  • Probiotics / therapeutic use*
  • Refrigeration
  • Temperature
  • Time Factors
  • Treatment Outcome