Ca2+ uptake during capacitation of mouse spermatozoa and the effect of an anion transport inhibitor on Ca2+ uptake

Mol Reprod Dev. 1990 May;26(1):63-8. doi: 10.1002/mrd.1080260110.

Abstract

With a specially constructed chamber, Ca2+ uptake by mouse spermatozoa was monitored continuously during capacitation and the acrosome reaction. It was shown, using calcium ion-selective microelectrodes, that there was an initial uptake of Ca2+ by spermatozoa undergoing capacitation. Such net transport was also promoted by the divalent cation ionophores A23187 or ionomycin. An anion inhibitor, SITS, produced dose-dependent inhibition of Ca2+ uptake. This inhibitor reduced the incidence of capacitation as revealed by a reduction in the B pattern by chlortetracycline (CTC) assay and thus inhibited fertilization, suggesting that anions are involved in calcium uptake in mouse spermatozoa.

MeSH terms

  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology*
  • Acrosome / metabolism
  • Animals
  • Biological Transport / drug effects
  • Calcimycin / pharmacology
  • Calcium / metabolism*
  • Female
  • Fertilization
  • Ionomycin / pharmacology
  • Male
  • Mice
  • Sperm Capacitation* / drug effects
  • Spermatozoa / drug effects
  • Spermatozoa / metabolism*
  • Stilbenes / pharmacology*

Substances

  • Stilbenes
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
  • Calcimycin
  • Ionomycin
  • Calcium