Expression analysis of five maize MAP kinase genes in response to various abiotic stresses and signal molecules

Abstract

Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules in eukaryotes. Plant MAPK cascades are complicated networks and play vital roles in signal transduction induced by biotic and abiotic stresses. In this paper, expression patterns of MAPKs in maize roots treated with low-temperature, osmotic stresses, wounding, plant hormones and UV-C irradiation were investigated. Semi-quantitative RT-PCR reveals that the expression of MAPKs in maize roots which treated with low-temperature in light or in low light are inducible. The expression patterns of MAPKs in maize roots with treatments of CaCl2, SA, GA and wounding are approximately the same. A detailed time course experiment shows that the expression patterns of ZmSIMK are different with treatments of PEG and NaCl, respectively. These results suggest that the expression patterns of MAPKs are complicated and the signal pathways are interlaced into a network in maize roots.