Increased Expression of Guanylate Binding protein-1 in Lesional Skin of Patients With Cutaneous Lupus Erythematosus

Exp Dermatol. 2011 Feb;20(2):102-6. doi: 10.1111/j.1600-0625.2010.01160.x. Epub 2010 Dec 2.

Abstract

The large GTPase human guanylate binding protein-1 (GBP-1) is a key mediator of angiostatic effects of inflammation and is induced by interferon (IFN)-α and IFN-γ in endothelial cells (ECs). The aim of this study was to investigate whether GBP-1 is a marker of skin lesions in patients with cutaneous lupus erythematosus (CLE). Western blotting revealed that GBP-1 was in vitro induced by IFN-α and -γ in primary keratinocytes obtained from healthy controls. Moreover, we found that this protein was expressed by keratinocytes and ECs in primary and ultraviolet (UV)-induced skin lesions from patients with various subtypes of CLE, when compared to non-lesional skin. No GBP-1 expression was noted in skin biopsy specimens 24 or 72 h after UV irradiation prior to lesion formation in patients with CLE or in healthy control specimens with or without UV irradiation. Initial findings suggest that GBP-1 is not expressed in other skin diseases with different inflammatory aetiology, such as atopic dermatitis. We conclude that GBP-1 expression is closely associated with skin lesions in patients with CLE, suggesting a contribution of GBP-1 in the pathogenesis of this disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biopsy
  • Case-Control Studies
  • Chemokine CXCL10 / metabolism
  • Chemokine CXCL9 / metabolism
  • Female
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Interferons / metabolism
  • Keratinocytes / metabolism*
  • Keratinocytes / pathology
  • Keratinocytes / radiation effects
  • Lupus Erythematosus, Cutaneous / metabolism*
  • Lupus Erythematosus, Cutaneous / pathology
  • Lupus Erythematosus, Cutaneous / radiotherapy
  • Male
  • Middle Aged
  • Myxovirus Resistance Proteins
  • Skin / metabolism*
  • Skin / pathology
  • Skin / radiation effects
  • Ultraviolet Therapy

Substances

  • Chemokine CXCL10
  • Chemokine CXCL9
  • GBP1 protein, human
  • Myxovirus Resistance Proteins
  • Interferons
  • GTP-Binding Proteins