Isolation and sequencing of rat liver bilirubin UDP-glucuronosyltransferase cDNA: possible alternate splicing of a common primary transcript

Biochem Biophys Res Commun. 1990 May 31;169(1):260-4. doi: 10.1016/0006-291x(90)91462-2.


A 1763-bp cDNA for rat liver bilirubin UDP-glucuronosyltransferase (UDPGT) was isolated. Bilirubin UDPGT activity was demonstrated by transfection of the pcDL1 vector carrying the cDNA into COS7 monkey kidney cells. The cDNA shares an identical 913-bp sequence (corresponding to the C-terminal 247 amino acid residues) with that for rat liver 3-methylcholanthrene-inducible 4-nitrophenol UDPGT including the locus where a -1 frameshift mutation was found in the 4-nitrophenol UDPGT cDNA from the jaundiced homozygous Gunn rat. The result suggests that both the UDPGTs are derived from a common primary-transcript and that the multiple defects of UDPGT isoenzymes observed in the homozygous Gunn rat may be produced by a single-mutated-locus after an alternative splicing of the 5' end region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bilirubin / metabolism
  • Cell Line
  • DNA / isolation & purification*
  • Glucuronosyltransferase / biosynthesis
  • Glucuronosyltransferase / genetics*
  • Haplorhini
  • Liver / enzymology*
  • Molecular Sequence Data
  • RNA Splicing
  • Rats
  • Transfection


  • DNA
  • Glucuronosyltransferase
  • Bilirubin

Associated data

  • GENBANK/M34007