A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting

Cell. 1990 Jun 15;61(6):1063-74. doi: 10.1016/0092-8674(90)90070-u.


Members of the Mx protein family promote interferon-inducible resistance to viral infection in mammals and act by unknown mechanisms. We identified an Mx-like protein in yeast and present genetic evidence for its cellular function. This protein, the VPS1 product, is essential for vacuolar protein sorting, normal organization of intracellular membranes, and growth at high temperature, implying that Mx-like proteins are engaged in fundamental cellular processes in eukaryotes. Vps1p contains a tripartite GTP binding motif, which suggests that binding to GTP is essential to its role in protein sorting. Vps1p-specific antibody labels punctate cytoplasmic structures that condense to larger structures in a Golgi-accumulating sec7 mutant; thus, Vps1p may associate with an intermediate organelle of the secretory pathway.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antiviral Agents / genetics
  • Base Sequence
  • Carrier Proteins / analysis
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • Fluorescent Antibody Technique
  • GTP-Binding Proteins / genetics*
  • GTP-Binding Proteins / metabolism
  • Genotype
  • Immunoblotting
  • Molecular Sequence Data
  • Mutation
  • Myxovirus Resistance Proteins
  • Protein Biosynthesis
  • Protein Processing, Post-Translational*
  • Proteins / genetics*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Sequence Homology, Nucleic Acid
  • Vertebrates
  • Vesicular Transport Proteins


  • Antiviral Agents
  • Carrier Proteins
  • Myxovirus Resistance Proteins
  • Proteins
  • Vesicular Transport Proteins
  • GTP-Binding Proteins
  • VPS1 protein, S cerevisiae

Associated data

  • GENBANK/M33315
  • GENBANK/X54316