Differentiation of mouse embryonic stem cells into cardiomyocytes via the hanging-drop and mass culture methods

Curr Protoc Stem Cell Biol. 2010 Dec;Chapter 1:Unit 1F.11. doi: 10.1002/9780470151808.sc01f11s15.

Abstract

Herein, we describe two protocols for the in vitro differentiation of mouse embryonic stem cells (mESCs) into cardiomyocytes. mESCs are pluripotent and can be differentiated into cells of all three germ layers, including cardiomyocytes. The methods described here facilitate the differentiation of mESCs into the different cardiac subtypes (atrial-, ventricular-, nodal-like cells). The duration of cell culture determines whether preferentially early- or late-developmental stage cardiomyocytes can be obtained preferentially. This approach allows the investigation of cardiomyocyte development and differentiation in vitro, and also allows for the enrichment and isolation of physiologically intact cardiomyocytes for transplantation purposes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotechnology / methods*
  • Cell Culture Techniques / methods
  • Cell Differentiation / physiology*
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / physiology*
  • Mice
  • Models, Biological
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / physiology*