Protective effects of Lactobacillus plantarum against epithelial barrier dysfunction of human colon cell line NCM460

World J Gastroenterol. 2010 Dec 7;16(45):5759-65. doi: 10.3748/wjg.v16.i45.5759.


Aim: To investigate the effects of Lactobacillus plantarum (L. plantarum) in the intestinal permeability and expression of tight junction (TJ) using the normal human colon cell line NCM460.

Methods: Paracellular permeability of NCM460 monolayers was determined by transepithelial electrical resistance and dextran permeability. Expression of TJ proteins in NCM460 cell monolayers was detected by Western blotting and quantitative real-time polymerase chain reaction.

Results: L. plantarum played an important role in increasing transepithelial electrical resistance and decreasing the permeability to macromolecules of NCM460 monolayers against the disruption caused by enteropathogenic Escherichia coli (E. coli) or enteroinvasive E. coli. L. plantarum also prevented the decrease in the expression of TJ proteins and F-actin in NCM460 cells.

Conclusion: L. plantarum can protect against dysfunction of NCM460 intestinal epithelial barrier caused by enteropathogenic E. coli or enteroinvasive E. coli, and thus can be a potential candidate of therapeutic agents for the treatment of intestinal diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Blotting, Western
  • Cell Line
  • Colon / cytology
  • Colon / metabolism
  • Colon / microbiology*
  • Dextrans / metabolism
  • Electric Impedance
  • Enteropathogenic Escherichia coli / pathogenicity
  • Escherichia coli / pathogenicity*
  • Humans
  • Lactobacillus plantarum / physiology*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Permeability
  • Probiotics*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tight Junctions / metabolism
  • Tight Junctions / microbiology*
  • Time Factors


  • Actins
  • Dextrans
  • Membrane Proteins
  • RNA, Messenger