Label-free biosensors based on aptamer-modified graphene field-effect transistors

J Am Chem Soc. 2010 Dec 29;132(51):18012-3. doi: 10.1021/ja108127r. Epub 2010 Dec 3.


A label-free immunosensor based on an aptamer-modified graphene field-effect transistor (G-FET) is demonstrated. Immunoglobulin E (IgE) aptamers with an approximate height of 3 nm were successfully immobilized on a graphene surface, as confirmed by atomic force microscopy. The aptamer-modified G-FET showed selective electrical detection of IgE protein. From the dependence of the drain current variation on the IgE concentration, the dissociation constant was estimated to be 47 nM, indicating good affinity and the potential for G-FETs to be used in biological sensors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Biosensing Techniques*
  • Graphite / chemistry*
  • Immunoglobulin E / analysis*
  • Microscopy, Atomic Force
  • Transistors, Electronic*


  • Aptamers, Nucleotide
  • Immunoglobulin E
  • Graphite