Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE

Am J Pathol. 1990 Jun;136(6):1229-33.


We have demonstrated inducible expression of the mRNA encoding the monocyte chemoattractant MCP-1, the human homolog of the JE gene, in endothelial cells within 3 hours of treatment with IL-1 beta and tumor necrosis factor. IFN-gamma also induced expression of this mRNA after 24 hours, but to a lesser extent. MCP-1/JE protein steadily accumulated in the medium of endothelial cells during a 48-hour exposure to IL-1 beta. Medium conditioned by IL-1 beta-treated endothelial cells contained monocyte chemoattractant activity that was immunoadsorbed by anti-MCP-1 antibodies. These results suggest that endothelial cells secrete a monocyte chemoattractant, MCP-1/JE, in response to inflammatory mediators, and thus may contribute to the accumulation of monocytes at sites of inflammation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Factors / pharmacology*
  • Cells, Cultured
  • Chemokine CCL2
  • Chemotactic Factors / genetics
  • Chemotactic Factors / metabolism*
  • Chemotaxis / physiology
  • Culture Media / pharmacology
  • Cytokines
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / metabolism
  • Endothelium, Vascular / physiology
  • Gene Expression / drug effects
  • Humans
  • Interferon-gamma / pharmacology
  • Interleukin-1 / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology


  • Biological Factors
  • Chemokine CCL2
  • Chemotactic Factors
  • Culture Media
  • Cytokines
  • Interleukin-1
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma