Abstract
The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 - 2 x 10(-10) cm2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Biological Transport
-
Cell Survival / drug effects
-
Electrophoresis, Polyacrylamide Gel
-
Endocytosis
-
Fluorescein-5-isothiocyanate
-
Fluoresceins
-
Fluorescent Dyes
-
Hemagglutination Tests
-
Humans
-
Kinetics
-
Liver Neoplasms, Experimental
-
Macromolecular Substances
-
Protein Binding
-
Rats
-
Ricin / isolation & purification
-
Ricin / metabolism*
-
Ricin / pharmacology
-
Thiocyanates
-
Tumor Cells, Cultured / cytology
-
Tumor Cells, Cultured / drug effects
-
Tumor Cells, Cultured / metabolism
Substances
-
Fluoresceins
-
Fluorescent Dyes
-
Macromolecular Substances
-
Thiocyanates
-
Ricin
-
Fluorescein-5-isothiocyanate