Binding and internalization of ricin labelled with fluorescein isothiocyanate

Biochem Biophys Res Commun. 1990 Jun 15;169(2):602-9. doi: 10.1016/0006-291x(90)90373-u.

Abstract

The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 - 2 x 10(-10) cm2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cell Survival / drug effects
  • Electrophoresis, Polyacrylamide Gel
  • Endocytosis
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Fluorescent Dyes
  • Hemagglutination Tests
  • Humans
  • Kinetics
  • Liver Neoplasms, Experimental
  • Macromolecular Substances
  • Protein Binding
  • Rats
  • Ricin / isolation & purification
  • Ricin / metabolism*
  • Ricin / pharmacology
  • Thiocyanates
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism

Substances

  • Fluoresceins
  • Fluorescent Dyes
  • Macromolecular Substances
  • Thiocyanates
  • Ricin
  • Fluorescein-5-isothiocyanate