Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection

Virol J. 2010 Dec 6;7:361. doi: 10.1186/1743-422X-7-361.

Abstract

Background: We compared the diagnostic accuracy and reproducibility of commercially available NS1-based dengue tests and explored factors influencing their sensitivities.

Methods: Paired analysis of 310 samples previously characterized as positive (n = 218) and negative (n = 92) for viral isolation and/or RT-PCR and/or IgM seroconversion. Masked samples were tested by two observers with Platelia™ Dengue NS1 Ag, second generation Pan-E™ Dengue Early ELISA, SD Dengue NS1 Ag ELISA, Dengue NS1 Ag STRIP™, and SD BIOLINE™ Dengue Duo (NS1/IgM/IgG).

Results: SD BIOLINE™ NS1/IgM/IgG had the highest sensitivity (80.7% 95%CI 75-85.7) with likelihood ratios of 7.4 (95%CI 4.1-13.8) and 0.21 (95%CI 0.16-0.28). The ELISA-format tests showed comparable sensitivities; all below 75%. STRIP™ and SD NS1 had even lower sensitivities (<65%). The sensitivities significantly decreased in samples taken after 3 days of fever onset, in secondary infections, viral serotypes 2 and 4, and severe dengue. Adding IgM or IgG to SD NS1 increased its sensitivity in all these situations.

Conclusions: The simultaneous detection of NS1/IgM/IgG would be potentially useful for dengue diagnosis in both endemic and non endemic areas. A negative result does not rule out dengue. Further studies are required to assess the performance and impact of early laboratory diagnosis of dengue in the routine clinical setting.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Viral / blood*
  • Child
  • Child, Preschool
  • Dengue / diagnosis*
  • Diagnostic Tests, Routine / methods*
  • Female
  • Humans
  • Immunoassay / methods
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Reagent Kits, Diagnostic*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viral Nonstructural Proteins / blood*
  • Virology / methods*
  • Young Adult

Substances

  • Antibodies, Viral
  • Reagent Kits, Diagnostic
  • Viral Nonstructural Proteins