Three-dimensional analysis of combination effect of ellagitannins and acyclovir on herpes simplex virus types 1 and 2

Antiviral Res. 2011 Feb;89(2):174-81. doi: 10.1016/j.antiviral.2010.11.014. Epub 2010 Dec 4.


The effects of combinations of three nonahydroxyterphenoyl-bearing C-glucosidic ellagitannins (castalagin, vescalagin and grandinin) with acyclovir (ACV) on the replication of type-1 and type-2 herpes simplex viruses in MDBK cells were tested by the focus-forming units reduction test. Ellagitannins included in these combinations possess a high individual antiviral activity: selectivity index of castalagin and vescalagin versus HSV-1 was similar to that of ACV, and relatively lower against HSV-2. The three-dimensional analytical approach of Prichard and Shipman was used to evaluate the impact of drug-drug interactions. The combination effects of ellagitannins with acyclovir were markedly synergistic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / pharmacology*
  • Animals
  • Antiviral Agents / pharmacology*
  • Cattle
  • Cell Line
  • Drug Synergism
  • Herpesvirus 1, Human / drug effects*
  • Herpesvirus 2, Human / drug effects*
  • Hydrolyzable Tannins / pharmacology*
  • Microbial Sensitivity Tests / methods
  • Virus Replication / drug effects


  • Antiviral Agents
  • Hydrolyzable Tannins
  • Acyclovir