DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement

Dev Cell. 2010 Dec 14;19(6):913-9. doi: 10.1016/j.devcel.2010.11.010.

Abstract

Centrioles form cilia and centrosomes, organelles whose dysfunction is increasingly linked to human disease. Centriole duplication relies on a few conserved proteins (ZYG-1/Sak/Plk4, SAS-6, SAS-5/Ana2, and SAS-4), and is often initiated by the formation of an inner "cartwheel" structure. Here, we show that overexpressed Drosophila Sas-6 and Ana2 coassemble into extended tubules (SAStubules) that bear a striking structural resemblance to the inner cartwheel of the centriole. SAStubules specifically interact with centriole proximal ends, but extra DSas-6/Ana2 is only recruited onto centrioles when Sak/Plk4 kinase is also overexpressed. This extra centriolar DSas-6/Ana2 induces centriole overduplication and, surprisingly, increased centriole cohesion. Intriguingly, we observe tubules that are structurally similar to SAStubules linking the engaged centrioles in normal wild-type cells. We conclude that DSas-6 and Ana2 normally cooperate to drive the formation of the centriole inner cartwheel and that they promote both centriole duplication and centriole cohesion in a Sak/Plk4-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Centrioles / metabolism*
  • Centrioles / ultrastructure*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development*
  • Drosophila melanogaster / metabolism*
  • Gene Expression
  • Genes, Insect
  • Humans
  • Male
  • Microscopy, Electron, Transmission
  • Protein Multimerization
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Spermatocytes / metabolism
  • Spermatocytes / ultrastructure

Substances

  • Ana2 protein, Drosophila
  • Asl protein, Drosophila
  • Cell Cycle Proteins
  • Drosophila Proteins
  • Sas-6 protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • Sak protein, Drosophila