Drosophila Krüppel protein is a transcriptional repressor

Nature. 1990 Jul 5;346(6279):76-9. doi: 10.1038/346076a0.


Krüppel (Kr), one of the zygotically active Drosophila segmentation genes, is expressed in a restricted domain during the blastoderm stage of embryogenesis and is involved in the control of development of the thoracic and abdominal segments of the fly. Kr encodes a polypeptide containing DNA-binding zinc-finger motifs, disruptions of which yield Kr mutants. We have assayed the transcriptional activities of wild-type Kr protein as well as Lac repressor/Kr fusion proteins in HeLa and CV-1 cells. Wild-type Kr and a Lac-Kr chimaeric protein repressed transcription from reporter promoters in which a consensus Kr binding site derived from sequences within the even-skipped promoter had been inserted in an upstream position. We mapped the repression function of Kr to an alanine-rich amino-terminal region of the protein, as a Lac/Kr fusion protein containing only amino acids 26-110 of Kr repressed transcription from a reporter promoter containing upstream lac operators. This demonstrates that the DNA-binding and repression activities of the Kr protein are distinct. These data are consistent with genetic evidence that Kr represses even-skipped and hunchback expression, and suggest that Kr is a negative regulator of transcription in Drosophila.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA-Binding Proteins / genetics
  • Drosophila Proteins
  • Drosophila melanogaster / genetics*
  • Juvenile Hormones / genetics*
  • Kruppel-Like Transcription Factors
  • Metalloproteins / genetics
  • Molecular Sequence Data
  • Mutation
  • Recombinant Proteins
  • Repressor Proteins / genetics*
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • DNA-Binding Proteins
  • Drosophila Proteins
  • Juvenile Hormones
  • Kr protein, Drosophila
  • Kruppel-Like Transcription Factors
  • Metalloproteins
  • Recombinant Proteins
  • Repressor Proteins
  • Transcription Factors