Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O60

Olga G Ovchinnikova; Nina A Kocharova; Angelina A Parkhomchuk; Magdalena Bialczak-Kokot; Alexander S Shashkov; Yuriy A Knirel; Antoni Rozalski

doi:10.1016/j.carres.2010.11.016

Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O60

Carbohydr Res. 2011 Feb 1;346(2):377-80. doi: 10.1016/j.carres.2010.11.016. Epub 2010 Nov 19.

Authors

Olga G Ovchinnikova¹, Nina A Kocharova, Angelina A Parkhomchuk, Magdalena Bialczak-Kokot, Alexander S Shashkov, Yuriy A Knirel, Antoni Rozalski

Affiliation

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. olga.ovchinnikova@gmail.com

PMID: 21146157
DOI: 10.1016/j.carres.2010.11.016

Abstract

An O-polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O60 and studied by sugar and methylation analyses as well as (1)H and (13)C NMR spectroscopy, including 2D ROESY and (1)H,(13)C HMBC experiments in D(2)O and a ROESY experiment in a 9:1 H(2)O-D(2)O mixture to reveal correlations for NH protons. It was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of d-glucuronic acid with l-serine and has the following structure: The O-antigen studied is structurally and serologically closely related to the O-antigen of Proteus vulgaris O44.

MeSH terms

Carbohydrate Sequence
Molecular Sequence Data
O Antigens / chemistry*
Providencia / immunology*

Substances

O Antigens