Brainstem Hap1-Ahi1 is involved in insulin-mediated feeding control

FEBS Lett. 2011 Jan 3;585(1):85-91. doi: 10.1016/j.febslet.2010.11.059. Epub 2010 Dec 10.

Abstract

The function of the brainstem Hap1-Ahi1 complex in the regulation of feeding behavior was investigated. When mice were fasted or treated with 2-deoxy-D-glucose (2-DG), Hap1-Ahi1 was significantly upregulated. By using streptozotocin (STZ) to decrease the circulating insulin in mice, Hap1-Ahi1 was significantly increased. Furthermore, intra-brain injection of insulin decreased the expression of Hap1-Ahi1 in the brainstem. Moreover, when we knocked down the expression of brainstem Hap1 by RNAi, the mice showed decreased food intake and lower body weights. Collectively, our results indicate that the Hap1-Ahi1 complex in the brainstem works as a sensor for insulin signals in feeding control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport
  • Animals
  • Blood Glucose / metabolism
  • Blotting, Western
  • Brain Stem / drug effects
  • Brain Stem / metabolism*
  • Cell Line, Tumor
  • Deoxyglucose / pharmacology
  • Diabetes Mellitus, Experimental / blood
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / metabolism
  • Eating / drug effects*
  • Fasting
  • Gene Expression Regulation / drug effects
  • Hypoglycemic Agents / pharmacology
  • Immunohistochemistry
  • Insulin / pharmacology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Multiprotein Complexes / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Protein Binding
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Solitary Nucleus / metabolism

Substances

  • Adaptor Proteins, Vesicular Transport
  • Ahi1 protein, mouse
  • Blood Glucose
  • Hap1 protein, mouse
  • Hypoglycemic Agents
  • Insulin
  • Multiprotein Complexes
  • Nerve Tissue Proteins
  • Proto-Oncogene Proteins
  • Deoxyglucose