MIH supplementation strategies: prospective clinical and laboratory trial

J Dent Res. 2011 Mar;90(3):371-6. doi: 10.1177/0022034510388036. Epub 2010 Dec 13.


The use of calcium-phosphate casein on hypomineralized molars (molar incisor hypomineralization, MIH) has been proposed but not clinically investigated. Qualitative and quantitative effects of supplementation with a calcium-phosphate casein product on MIH molars were monitored over a period of three years. Molar replicas, minimally invasive biopsies and their SEM microphotographs, plus ESEM/EDX semi-quantitative peaks of elements present in affected enamel were evaluated. Mineralization, morphology, and porosity appeared markedly improved, with calcium and phosphate levels reaching almost normal levels at three years' follow-up. The hypothesis tested was rejected, since calcium-phosphate casein improved enamel morphology in vivo.

Publication types

  • Clinical Trial

MeSH terms

  • Administration, Topical
  • Caseins / administration & dosage
  • Caseins / therapeutic use*
  • Child
  • Dental Enamel / pathology*
  • Dental Enamel / ultrastructure
  • Dental Enamel Hypoplasia / drug therapy*
  • Humans
  • Microscopy, Electron, Scanning
  • Minerals / analysis
  • Prospective Studies
  • Replica Techniques
  • Spectrometry, X-Ray Emission


  • Caseins
  • Minerals
  • casein phosphopeptide-amorphous calcium phosphate nanocomplex