Raman spectroscopy and related techniques in biomedicine

Sensors (Basel). 2010;10(3):1871-89. doi: 10.3390/s100301871.


In this review we describe label-free optical spectroscopy techniques which are able to non-invasively measure the (bio)chemistry in biological systems. Raman spectroscopy uses visible or near-infrared light to measure a spectrum of vibrational bonds in seconds. Coherent anti-Stokes Raman (CARS) microscopy and stimulated Raman loss (SRL) microscopy are orders of magnitude more efficient than Raman spectroscopy, and are able to acquire high quality chemically-specific images in seconds. We discuss the benefits and limitations of all techniques, with particular emphasis on applications in biomedicine--both in vivo (using fiber endoscopes) and in vitro (in optical microscopes).

Keywords: CARS microscopy; Raman imaging; Raman spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Line, Tumor
  • Histocytochemistry
  • Humans
  • Microscopy*
  • Principal Component Analysis
  • Single-Cell Analysis
  • Spectrum Analysis, Raman*