Silver nanocluster aptamers: in situ generation of intrinsically fluorescent recognition ligands for protein detection

Chem Commun (Camb). 2011 Feb 28;47(8):2294-6. doi: 10.1039/c0cc03711g. Epub 2010 Dec 9.

Abstract

We have synthesized an intrinsically fluorescent recognition ligand that combines the high fluorescence quantum yield (>50%) of oligonucleotide templated AgNCs with the specificity and strong binding affinity of DNA aptamers for their target proteins, to develop a new strategy for detection of specific proteins.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Humans
  • Ligands
  • Nanostructures / chemistry*
  • Oligonucleotides / chemistry
  • Quantum Theory
  • Silver / chemistry*
  • Spectrometry, Fluorescence / methods*
  • Thrombin / analysis*

Substances

  • Aptamers, Nucleotide
  • Ligands
  • Oligonucleotides
  • Silver
  • Thrombin