Intravital fluorescence microscopic molecular imaging of atherosclerosis

Methods Mol Biol. 2011;680:131-40. doi: 10.1007/978-1-60761-901-7_9.

Abstract

Atherosclerosis is a lipid deposition and inflammatory disease that results in considerable morbidity and mortality worldwide. Advances in molecular imaging, particularly near-infrared fluorescence imaging, are now enabling the in vivo study of fundamental biological processes that govern atherogenesis and its complications. Here we describe applications of near-infrared fluorescence reporter technology and intravital fluorescence microscopy to elucidate important biological processes in atherosclerosis in vivo.

MeSH terms

  • Animals
  • Atherosclerosis / pathology*
  • Dextrans
  • Fluorescein-5-isothiocyanate / analogs & derivatives
  • Mice
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods*

Substances

  • Dextrans
  • fluorescein isothiocyanate dextran
  • Fluorescein-5-isothiocyanate