Abstract
PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium-catalyzed Buchwald–Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Cell Survival
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Coumarins / chemical synthesis*
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Coumarins / metabolism*
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Fluorescent Dyes / chemical synthesis*
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Fluorescent Dyes / metabolism*
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Humans
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Molecular Imaging
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Proteins / metabolism*
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Staining and Labeling / methods*
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Substrate Specificity
Substances
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Coumarins
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Fluorescent Dyes
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Proteins
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7-aminocoumarin