The Upstream Region of the IPNS Gene Determines Expression During Secondary Metabolism in Aspergillus Nidulans

Gene. 1990 Apr 30;89(1):109-15. doi: 10.1016/0378-1119(90)90212-a.

Abstract

We have constructed a translational fusion between the isopenicillin-N-synthetase-encoding gene (IPNS) of Aspergillus nidulans and the lacZ gene of Escherichia coli. Recombinant strains carrying a single copy of the fusion integrated at the IPNS locus produced beta-galactosidase (beta Gal) during secondary metabolism. Integration of the fusion at the argB locus results in a situation in which the only 5'-flanking sequences of the IPNS gene upstream from the chimeric fused gene are those included in the transforming plasmid. Such a strain still expresses beta Gal activity during secondary metabolism, showing that a DNA fragment including sequences of the IPNS gene from nt -2000 to +35 (relative to the translation start codon) still contains sufficient information to drive expression of the fusion gene during secondary metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus nidulans / genetics*
  • Aspergillus nidulans / metabolism
  • Blotting, Southern
  • Blotting, Western
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Escherichia coli / genetics
  • Genes, Fungal*
  • Kinetics
  • Molecular Weight
  • Oxidoreductases / genetics*
  • Oxidoreductases / isolation & purification
  • Plasmids
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Restriction Mapping
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • DNA, Fungal
  • Recombinant Fusion Proteins
  • Oxidoreductases
  • isopenicillin N synthetase
  • beta-Galactosidase