Human papillomavirus DNA and P16(INK4A) expression in concurrent esophageal and gastric cardia cancers

World J Gastroenterol. 2010 Dec 14;16(46):5901-6. doi: 10.3748/wjg.v16.i46.5901.


Aim: To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16(INK4A) protein expression.

Methods: Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16). The expression of P16(INK4A) protein was detected using immunohistochemistry.

Results: Among the CC specimens, HPV16-DNA was found in eight cases of esophageal squamous cell carcinoma (ESCC) and five cases of gastric cardia adenocarcinoma (GCA), respectively (47% vs 29%), and two of both ESCC and GCA. P16(INK4A) was highly expressed in both ESCC and GCA. In the HPV-associated positive CC, higher P16(INK4A) expression was observed in the GCA than in the ESCC (75% vs 25%, P < 0.05).

Conclusion: HPV16 as a correlated risk factor may play an important role in the development of ESCC and GCA. P16(INK4A) may be a screening index in the HPV-associated carcinoma of gastric cardia.

MeSH terms

  • Aged
  • Cardia / pathology*
  • Cyclin-Dependent Kinase Inhibitor p16 / genetics
  • Cyclin-Dependent Kinase Inhibitor p16 / metabolism*
  • DNA, Viral / genetics*
  • Esophageal Neoplasms / metabolism
  • Esophageal Neoplasms / pathology
  • Esophageal Neoplasms / virology*
  • Female
  • Human papillomavirus 16 / genetics*
  • Humans
  • Male
  • Middle Aged
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / virology*


  • Cyclin-Dependent Kinase Inhibitor p16
  • DNA, Viral