Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy

Biochem Biophys Res Commun. 2011 Jan 14;404(2):689-94. doi: 10.1016/j.bbrc.2010.12.043. Epub 2010 Dec 13.


The topography and mechanical properties of single B-lymphoma cells have been investigated by atomic force microscopy (AFM). With the assistance of microfabricated patterned pillars, the surface topography and ultrastructure of single living B-lymphoma cell were visualized by AFM. The apoptosis of B-lymphoma cells induced by rituximab alone was observed by acridine orange/ethidium bromide (AO/EB) double fluorescent staining. The rituximab-induced changes of mechanical properties in B-lymphoma cells were measured dynamically and the results showed that B-lymphoma cells became dramatically softer after incubation with rituximab. These results can improve our understanding of rituximab'effect and will facilitate the further investigation of the underlying mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal, Murine-Derived / pharmacology*
  • Antineoplastic Agents / pharmacology*
  • Cell Line, Tumor
  • Cell Membrane / diagnostic imaging
  • Cell Membrane / drug effects
  • Humans
  • Lymphoma, B-Cell / ultrastructure*
  • Microscopy, Atomic Force*
  • Rituximab
  • Ultrasonography


  • Antibodies, Monoclonal, Murine-Derived
  • Antineoplastic Agents
  • Rituximab