A Sandwich Enzyme Immunoassay for Bovine Interferon-Gamma and Its Use for the Detection of Tuberculosis in Cattle

Aust Vet J. 1990 Apr;67(4):134-7. doi: 10.1111/j.1751-0813.1990.tb07730.x.

Abstract

An in vitro cellular assay for bovine tuberculosis has recently been developed. This assay detects gamma-interferon released in response to specific antigen in a whole blood culture system. The bio-assay previously described for the detection of bovine gamma-interferon (IFN-gamma) has now been replaced with a sandwich enzyme immunoassay (EIA) which utilises two monoclonal antibodies to bovine IFN-gamma. The EIA detects less than 25pg/ml of recombinant bovine IFN-gamma and is specific for biologically active bovine IFN-gamma; and does not detect bovine alpha or beta interferon. IFN-gamma from sheep, goat and buffalo, but not from pig, deer or man, are also recognised by the EIA. The bovine IFN-gamma EIA when used in conjunction with the whole blood culture system has resulted in a simple, rapid and sensitive in vitro assay for specific cell mediated immune responsiveness to M. bovis infection in cattle.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Biological Assay
  • Cattle
  • Female
  • Immunoenzyme Techniques*
  • Interferon-gamma / analysis*
  • Interferon-gamma / immunology
  • Predictive Value of Tests
  • Random Allocation
  • Species Specificity
  • Tuberculin / immunology
  • Tuberculosis, Bovine / diagnosis*

Substances

  • Antibodies, Monoclonal
  • Tuberculin
  • Interferon-gamma