E6 and E7 proteins from different beta-papillomaviruses types do not interfere in UVB-induced apoptosis of HaCaT keratinocytes

Exp Dermatol. 2011 Jan;20(1):71-3. doi: 10.1111/j.1600-0625.2010.01197.x.

Abstract

Beta-papillomaviruses (beta-HPV) have been linked to the development of skin cancer in humans. Because both E6 and E7 proteins from beta-HPV have been involved in the potential carcinogenicity of these viruses, we investigated their role on UVB-induced apoptosis in HaCaT cell line. HaCaT cells have been transduced with both E6/E7 using a retroviral system and treated with PRIMA-1. Apoptosis was assessed by flow cytometry to measure mitochondrial membrane potential and DNA fragmentation. HaCat keratinocytes transduced with both E6 and E7 genes of seven beta-HPV types (HPV5, HPV8, HPV14, HPV24, HPV36, HPV38 and HPV49) did not demonstrate any inhibition of UVB-induced apoptosis, even after p53 reactivation through PRIMA-1. Our data suggest that the expression of E6 and E7 exert different modulatory effects on UVB-induced apoptosis according to beta-HPV types and to the cellular genetic context.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Apoptosis / radiation effects*
  • Aza Compounds / pharmacology
  • Betapapillomavirus / pathogenicity*
  • Betapapillomavirus / physiology
  • Bridged Bicyclo Compounds, Heterocyclic / pharmacology
  • Cell Line
  • Cocarcinogenesis
  • Humans
  • Keratinocytes / pathology
  • Keratinocytes / radiation effects*
  • Keratinocytes / virology*
  • Oncogene Proteins, Viral / physiology*
  • Skin Neoplasms / etiology
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / physiology
  • Ultraviolet Rays / adverse effects*

Substances

  • Aza Compounds
  • Bridged Bicyclo Compounds, Heterocyclic
  • Oncogene Proteins, Viral
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • 2,2-bis(hydroxymethyl)-1-azabicyclo(2,2,2,)octan-3-one