Inclusion of the Central Exon of Parvovirus B19 Precursor mRNA Is Determined by Multiple Splicing Enhancers in Both the Exon and the Downstream Intron

J Virol. 2011 Mar;85(5):2463-8. doi: 10.1128/JVI.01708-10. Epub 2010 Dec 15.

Abstract

Alternative splicing of the precursor mRNA (pre-mRNA) of human parvovirus B19 (B19V) plays a key role in posttranscriptional regulation of B19V gene expression. We report that the central exon of the B19V pre-mRNA is defined by three GAA motif-containing exonic splicing enhancers and a G/GU-rich intronic splicing enhancer that lies adjacent to the second donor site. Moreover, targeting of morpholino antisense oligonucleotides to the two splicing enhancers surrounding the second donor site led to a significant reduction in splicing at this donor site during B19V infection of permissive CD36(+) erythroid progenitor cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alternative Splicing*
  • Base Sequence
  • Enhancer Elements, Genetic*
  • Erythema Infectiosum / virology
  • Erythroid Precursor Cells / virology
  • Exons*
  • Humans
  • Introns*
  • Molecular Sequence Data
  • Parvovirus B19, Human / genetics*
  • RNA Precursors / genetics*
  • RNA, Messenger / genetics
  • RNA, Viral / genetics*

Substances

  • RNA Precursors
  • RNA, Messenger
  • RNA, Viral