Recognition of keratinocytes by cytotoxic T cells specific for conventional HLA class-I alloantigen

J Invest Dermatol. 1990 Aug;95(2):224-8. doi: 10.1111/1523-1747.ep12478064.


This study analyzed whether human keratinocytes (KC) express conventional HLA class-I molecules as detected by class-I-specific cytotoxic T lymphocytes (CTL), and whether exposure of KC to interferon-gamma (IFN-g) is required for CTL recognition. Basal KC grown in serum-free medium and exposed to recombinant IFN-g for 24-96 h were used as targets in 51Cr-release assays. Target-cell susceptibilities to lysis were compared by analyzing the lytic unit (LU) activity of a given CTL population against IFN-g-treated and untreated KC. CTL effectors were cloned from alloantigen-primed cultures by limiting dilution in the presence of antigenic B lymphoblastoid cells (BCLL) and IL-2. These T-cell clones lysed appropriate BCLL and PHA blasts but not third-party BCLL or K562. Lysis of antigenic BCLL was specifically blocked by antibodies against CD3 or class-I antigens. Specificity of the clones for conventional class-I antigen was demonstrated by cytotoxicity tests employing a panel of HLA-typed BCLL. The clones specifically lysed KC syngeneic with the original effector immunogen, and lysis was also blocked by anti-class-I antibodies. The effect of IFN-g treatment was to increase KC susceptibility to lysis by these clones. From 3-25 times more LU were measured against IFN-g-treated KC than against nontreated KC, and the degree of enhancement was similar for KC treated with concentrations of IFN-g ranging from 2.5-200 U/ml. This effect of IFN-g treatment on KC lysis by CTL, which was detected after only 24 h at all doses tested, emphasizes the potential role of IFN-g in enhancing CTL-mediated antiviral epidermal immunity and in exacerbating epidermal disease mediated by specific lytic T cells. In addition, the finding that normal human KC can be recognized by MHC class-I-specific CTL demonstrates that KC do express conventional class-I-antigens and that KC lysis by CTL can occur independently of exogenous cytokines.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • B-Lymphocytes
  • Cell Line
  • Cells, Cultured
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Interferon-gamma / pharmacology
  • Interleukin-2 / pharmacology
  • Isoantigens / immunology*
  • Keratinocytes / drug effects
  • Keratinocytes / immunology*
  • Recombinant Proteins / pharmacology
  • T-Lymphocytes, Cytotoxic / immunology*


  • Histocompatibility Antigens Class I
  • Interleukin-2
  • Isoantigens
  • Recombinant Proteins
  • Interferon-gamma