Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

Opt Lett. 2010 Dec 15;35(24):4102-4. doi: 10.1364/OL.35.004102.


We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dermis / cytology
  • Epidermal Cells
  • Holography / methods*
  • Image Processing, Computer-Assisted
  • Mice
  • Microscopy / methods*
  • Molecular Imaging / methods*