Antitumor effect of endostatin overexpressed in C6 glioma cells is associated with the down-regulation of VEGF

Int J Oncol. 2011 Feb;38(2):465-71. doi: 10.3892/ijo.2010.871. Epub 2010 Dec 14.


Endostatin is an anti-angiogenic agent that blocks endothelial cell proliferation, tumor growth, and metastasis. Several lines of direct evidence show that gliomas express high levels of endostatin. However, the anti-angiogenic activity and cellular mechanisms of endostatin from tumor cells have not been completely elucidated. In this study, we established C6 glioblastoma (GBM) xenografts in nude mice by subcutaneously injecting glioma cell lines, C6-null cells, and stable transfected-C6 cells overexpressing mock vector (C6-mock) and endostatin (C6-endo). We found that overexpression of endostatin in C6-endo cells significantly suppressed the expression of VEGF in tumor cells in vivo as well as in vitro. Furthermore, the tumor growth derived from C6-endo cells was inhibited. Our data demonstrate that endostatin could play a direct role in inhibiting tumor cells, and suggest that enhancing endostatin expression in gliomas could be an effective treatment strategy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Angiogenesis Inhibitors
  • Animals
  • Apoptosis / drug effects
  • Blotting, Western
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Disease Models, Animal
  • Down-Regulation
  • Endostatins / genetics*
  • Gene Expression
  • Genetic Vectors
  • Glioblastoma / metabolism*
  • Glioblastoma / pathology
  • Immunoenzyme Techniques
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / secondary
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Organophosphorus Compounds / pharmacology*
  • Rats
  • Vascular Endothelial Growth Factors / metabolism*


  • Angiogenesis Inhibitors
  • Endostatins
  • N-acetylcochinol-O-phosphate
  • Organophosphorus Compounds
  • Vascular Endothelial Growth Factors