Background: Antibodies against polymorphic structures on human neutrophil antigens (HNAs) play a role in alloimmune-mediated neutropenia and are the leading cause of antibody-mediated transfusion-related acute lung injury (TRALI). This study aimed to determine the frequencies of HNAs in the major Han ethnic group living in Guangdong Province, Southern China.
Study design and methods: A total of 493 healthy Chinese Han blood donors from Guangzhou were recruited. DNA samples were isolated and typed for all five HNA-1, -2, -3, -4, and -5 systems using allele-specific polymerase chain reaction approaches. Results were compared with available data from other Chinese cohorts and other Asian and Caucasian populations.
Results: In this cohort, the gene frequency for HNA-1a (0.667) was approximately twice that of HNA-1b (0.333). In contrast to Caucasian populations, HNA-1a represents the most frequent allele in the Chinese population. HNA-3 system genotyping revealed comparable frequencies for HNA-3a (0.738) and -3b (0.262) in Chinese and Caucasian populations. Homozygous HNA-3 bb individuals were found in 5.64% of our cohort. HNA-4 genotyping revealed no HNA-4 bb homozygous individuals. In contrast, HNA-5 bb homozygous individuals represented 2.43% of the population. Typing the HNA-2 system for the single-nucleotide polymorphism C42G showed that the C-allele (69%) is overrepresented and is associated with an increased number of HNA-2a-positive neutrophil subpopulations.
Conclusion: This study describes for the first time the frequencies of all HNA systems, including the newly identified HNA-3, within one cohort of Chinese Han population. Comparison with Caucasian populations may allow assessment of anti-HNA alloimmunization and estimation of alloimmune neutropenia and TRALI incidence in Chinese populations.
© 2010 American Association of Blood Banks.